PARR Hücre Parçalama Bombaları

PARR Hücre Parçalama Bombaları

4635 & 4639 Cell Disruption Vessels

4635 & 4639 Cell Disruption Vessels

A rapid and effective way to:

  • Homogenize cells and tissues
  • Release intact organelles
  • Prepare cell membranes
  • Release labile biochemicals
  • Produce uniform and repeatable homogenates without subjecting the sample to extreme chemical or physical stress.

How it Works

The principle of the method is quite simple. Large quantities of nitrogen are first dissolved in the cell under high pressure within a suitable pressure vessel. Then, when the gas pressure is suddenly released, the nitrogen comes out of the solution as expanding bubbles that stretch the membranes of each cell until they rupture and release the contents of the cell.

Parr Cell Disruption Vessels

Parr stainless steel vessels for processing cell suspensions by the nitrogen decompression method are made in several sizes with full opening heads and self sealing closures which can be handled easily on any laboratory bench without special tools or fixtures. Each vessel has two valves and a pressure gage: one valve for charging with nitrogen and the other for withdrawing the homogenate and discharging it through an attached delivery tube. All of the fittings as well as the vessel itself are made of stainless steel with polished surfaces for good corrosion resistance and freedom from contamination. The individual parts are easily cleaned and can be thoroughly sterilized.

Designs for Large and Small Samples

These vessels are made in five different sizes to accommodate samples ranging from 0.5 mL to 5 liters, The maximum charging capacity for each vessel is limited to two-thirds of the internal volume of the vessel, but smaller samples can be treated in any of these vessels by simply placing the sample in a beaker or test tube and positioning it under the dip tube within the vessel.

Easy Access to Vessel Chamber

Each of these vessels, except the small 45 mL size, has a split-ring closure — an exclusive Parr design which allows the vessel to be opened or closed easily without disturbing any of the fittings or connecting lines attached to the head. In this closure two ring sections slide into place from the sides of the vessel to lock the head in position while a self-sealing O-ring maintains a tight seal at all pressures. The ring sections are secured by a steel retaining band that is raised from the bottom of the vessel and anchored with a single, hand-tightened screw.

On the small, 45 mL vessel, a firm closure is obtained by simply turning down a knurled cap until it is hand tight. No wrenches or fixtures are required.

Safety Protection

All Parr Cell Disruption Vessels are designed to accommodate the full 2000 psig available from a commercial nitrogen cylinder. If higher pressures should accidentally develop, the 920 mL and larger vessels are protected by a safety rupture disc which will burst at approximately 3000 psig, well below the pressure at which any parts of the vessel would fail. A 3000 psi pressure gage and a 3000 psig rupture disc are standard but other gages and discs with lower pressure ratings can be furnished on special order.

Custom Modifications

Most cell disruption procedures can be handled readily in the 920 mL, 4635 Vessel with its customary fittings and attachments, yet applications may arise in which it will be desirable to modify a vessel to meet special requirements. If initial studies show a need for modifications, the user can return any vessel to the factory for such changes or additions as may be needed. Changes sometimes requested include:

Multiple sample capacity.

Up to three additional dip tubes and discharge valves can be installed in any vessel (except the 45 mL size). With these additional outlets and appropriate sample holders, as many as four samples can be processed independently and simultaneously in a single unit.

Special valves.

Valves with either larger or smaller orifices can be provided. Users processing large quantities of materials that have a tendency to plug the orifice of the standard throttling valve may want to substitute either a larger needle valve or a ball valve to permit faster flow rates or to avoid stoppage. Fine metering valves with smaller orifices and very precise control capabilities may be helpful when treating samples that must be processed under very exact and repeatable conditions. Such valves may also be desirable when working with very small samples.

Size conversion.

Since cylinders for the 920 and 1850 mL vessels are interchangeable and both vessels use the same head and closure, any user of a 4635 Vessel can increase the capacity of their unit to the 4636 size by simply purchasing a deeper cylinder (599HC22) and a longer dip tube (591HC5). A similar conversion can also be made from the one gallon vessel to the two gallon size for users interested in vaccine production or in similar large volume applications. These larger vessels can also be equipped with vigorous internal agitators to speed equilibrium times when processing large volumes of material.

Cell Disruption Vessels Selection Guide

Cell Disruption Vessels
Part No. 4635 4636 4637 4638 4639
Internal Volume 920 mL 1850 mL 1 Gallon 2 Gallon 45 mL
Maximum Sample Size 600 mL 1200 mL 2.5 L 5.0 L 30 mL
Maximum Working Pressure, psig 2200 2200 2200 2200 2200
Inside Diameter 3.75 Inches 3.75 Inches 6.0 Inches 6.0 Inches 1.5 Inches
Inside Depth 5.1 Inches 10.1 Inches 8.6 Inches 17.2 Inches 2.25 Inches
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