Biotron LiFlus GD

LiFlus GD

Autoclavable lab scale system with double vessels.

Space saving
Easy operation
Various sizes of vessels are interchangeable
Air-cooling method without water supplying line
Microprocessor based digital PID control

 liflusgd

FEAUTURES

The LiFlus GD fermentation system is a lab scale fermentor which is designed for multipurpose fermentation. Various fermentation schemes such as fedbatch and even the animal cell culture can be pertalined with the BioGM series. The monolithic LCD monitor is installed in the half tower size controller body and it can display all the measured values and control parameters. 4peristaltic pumps are installed in the controller body that acid, base, antifoam agent and media can be supplied to the fermentor. The various parameters for fermentation such as pH, DO, temperature and the rotating speed for the fermentor can be monitored and controlled by the controller and all the data and control parameters can be transmitted to the multiple fermentor controller system or computer system. The various components can be assembled in a fermentation system. The various size of single vessel with hot plate or double vessel with water bath can be selected. The agitation motor is installed on the top plate and the top driving mechanism can make the simple configuration and high reliability.

  • Various size of the single and double wall type vessel can be selected.
  • The top driving configuration with DC servo motor makes simple configuration of the head plate.
  • The rotating speed of the agitator can be modified and controlled from 50 to 1,200 rpm.
  • The digital PID control algorithm makes fast approach to the target temperature and stable temperature maintaining capability.
  • The pH value is compensated precisely with the temperature and the value of deadband for the peristaltic pump control can be adjusted.
  • The DO value is compensated with temperature, salinity and altitude to measure the exact value and the value of dead-band for the peristaltic pump control can be adjusted also.
  • The ring shape sparger is made of stainless steel and multiple hole helps uniform foam distribution.
  • The controller can be connected to the multiple monitoring and control system.
  • The measured data and control parameters can be saved and transmitted to the computer in Excel format.
  • The Li-ion battery is used to backup the data in the memory.
  • The four channel gas mixer can be attached to the LiFlus GD system.
  • The thermal printer can be attached to the controller to printout the realtime data.
  • It is very easy to connect with the various systems such as memory stick, printer, computer, BioG- and etc.


SPECIFICATIONS

Agitation Drive Top drive motor (mechanical drive)
 
Range 50~1,200 rpm
 
Sensor Magnetic hall sensor
 
Impellers 2-Rushton turbine impeller, 1-foam breaker
 
Indication Monolitic LCD display
Temperature Range From 3 above the cooling water temperature to 80
(accuracy 0.1)
 
Control Digital temperature controller
 
Sensor RTD(Pt-100)sensor
Aeration 2 Gas Mode Air, O2
 
4 Gas Mode Air, O2, CO2, N2,(in cell culture, in plant cell culture)
 
Air Flow, Electronic TMM or TMC
 
Sparger Ring sparger
 
Inlet Filter O.2×I air filter cartridge
Exhaust System Filter O.2×I air filter cartridge
 
Condenser SUS316
pH Setpoint / Display Range 2.0 ~ 12.0
 
Control Modes PID maintain with 0.01 pH of setpoint
 
Electrode Mettler Toledo or Hamilton electrode
DO Setpoint Range 0 ~ 200% 0.1
 
Display Range 0 ~ 200% 0.1
 
Control Modes PID
 
Electrode Mettler Toledo or Hamilton electrode
Control Cabinet Dmension 250 D x 350 W x 300 H(mm)
   
  Power 220V, 50/60Hz
Peristaltic Pumps Control 4 pumps each assignable to a wide variety of function for control of acid, base, foam and feed
Recording Output pH, DO, Foam, RS-232C
Heating Plate Electric Heater 400 watt
 
Dimension 250 D x 300 W x 100 H(mm)
 
Control PID

VESSELS

Single Vessel
Top Plate (pH, DO, anti-foam, Thermo well, Inoculation, Sampling 4point feeding ports)
Ports Cap, Blade, Foam Breaker, Pyrex Glass condenser, Ring type Sparger,
Removal Baffle Powerful DC Motor, Single Mechanical Seal, Pyrex Glass Vessel silicon Tube,
Vessel Packing, cold Finger Heating Plate (Heater, Water in/out ports)
* Please refer to the below for the specification/price according to volume & type of each item
* Price depends on the type, but some of items have the same price regardless of type.
Catalog Number Total Vol.
(working Vol.)

Inner Dia.
(mm)

Inner Heighta
(mm) 		Electrode Length
(mm) 		Min Vol.
(L) 		Impeller Diameter
(mm)		 D/P
(%)		 H:D
D H P
PF1G2SL02 1.5L (1L) 110 150 120 0.3 60 55 1.3 :1
PF1G2SL03 3L (2.5L) 130 220 120 0.5 60 45 1.7 :1
PF1G2SL05 5L (3L) 145 300 225 1.5 60 42 1.2 :1
PF1G2SL07 7L (5L) 170 310 225 3.2 72 45 1.7 :1
PF1G2SL10 10L (7L) 190 355 320 6 80 42 1.8 :1
   
Double Vessel
Pyrex Glass double Vessel Assembly with Vessel supporter Top Plate (pH,DO,Anti-foam, Thermo well,
Inoculation, Sampling 4poin feeding points). Ports, Blade, Foam Breaker Pyrex Glass Condenser,
Ring type Sparger, Removal Baffle Powerful DC Motor, Seal, Pyrex Glass Vessel Silicon Tube,
Vessel packing, Cold Finger Heating Plate(Heater, water in/out ports)
* Double vessel doesn't need Heating Plate but Water bath for exclusive use
Catalog Number Total Vol.
(working Vol.)

Inner Dia.
(mm)

Inner Heighta
(mm) 		Electrode Length
(mm) 		Min Vol.
(L) 		Impeller Diameter
(mm)		 D/P
(%)		 H:D
D H P
PF1G2DL02 1.5L (1L) 110 150 120 0.3 60 55 1.3 :1
PF1G2DL03 3L (2.5L) 130 220 120 0.5 60 45 1.7 :1
PF1G2DL05 5L (3L) 145 300 225 1.5 60 42 1.2 :1
PF1G2DL07 7L (5L) 170 310 225 3.2 72 45 1.7 :1
   
Bowl Vessel
Pyrex glass single Vessel with BOWL type low plate Top Plate (pH, DO, Anti-foam, Thermo well, Inoculation, Sampling 4 point feeding ports SUS ports Cap, Blade, Foam Breaker Pyrex Glass Condenser, Ring type sparger, Removal Baffle Powerful DC Motor, Single Mechanical Seal, Pyrex Glass Vessel Silicon Tube, Quick coupling, Vessel Packing Heating Plate (Heater, Water in/out ports) * Single/Double vessel has the range up to 10L, impossible for the bigger volume to control the Temp with Cold Finger. Bowel vessel is excellent at freezing vessel because the bottom plate is made of round SUES * BOWL Vessel doesn't need Heating Plate but Water Bath for exclusive use
Catalog Number Total Vol.
(working Vol.)

Inner Dia.
(mm)

Inner Heighta
(mm) 		Electrode Length
(mm) 		Min Vol.
(L) 		Impeller Diameter
(mm)		 D/P
(%)		 H:D
D H P
 
PF1G2DL07 10L (7L) 190 355 320 6 80 42 1.8 :1
PF1G2DL07 14L (10L) 190 500 425 6 80 42 2.6 :1
   

Animal cell Vessel (Depth filter setting)

Mass cultivation of animal cells has intensively been investigated even thought cell culture techniques have been developed for long times.

There have been several difficulties in maintaining large quantities of live cells, especially more than million of cells per m/ in over five liter vessel. Fragility of animal cell, sophisticated culture conditions less economical feasibility of being scaled-up were major bottle necks in idustrializing cell culture technologies to produce a large amount of pharmaceuticals, tPA, interferon, EPO and many other MAb.

 

 

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